The purifi cation of a valuable product or product library after a long and complex synthesis is a very important process. The Agilent Automated Purifi cation Software introduces an Open-Access-based solution for isolating substances, with automated focused gradient to simplify the preand post-purifi cation steps. This Technical Overview describes the easy steps to follow, from crude sample ana...

Drug metabolism and pharmacokinetics (DMPK) studies require the identifi cation of drug metabolites usually by mass spectrometry (MS), and in later stages of drug development, confi rmation of metabolite structures by NMR. To achieve this, purifi cation of metabolites may be required. In this Application Note, purifi cation of bupropion drug metabolites was achieved using Agilent Automated Puri...

Most studies conducted to test the allelopathic activity of plant parts have made use of water as solvent. However, the presence of polar, water-soluble substances, such as proteins and carbohydrates, tends to hamper the purifi cation of active compounds. In this study, we present a simple purifi cation procedure that separates the active fraction of the extract from the undesirable substances,...

scorpion venoms contain of variety of peptides toxic to mammals ، insects and crustaceans. toxic peptides are the main factors in scorpion venom causing toxicity, (their amount being 1-3%of total venom.). most of the scorpion toxins have been isolated from the venoms of scorpions in the family buthidae. the scorpion buthotus schach belonging to the buthidae family is widely found in the western...

Juvenile hormone esterase (JHE) from the serum of the cricket, Gryllus assimilis, was purifi ed to homogeneity in a four-step procedure involving polyethylene glycol precipitation, hydrophobic interaction FPLC, and ion exchange FPLC. This procedure could be completed in 4 days and resulted in a greater than 900-fold purifi cation with greater than 30% recovery. The purifi ed enzyme exhibited a ...

This Application Note used hen egg white lysozyme as a model protein for purifi cation and biophysical characterization of PEGylated proteins. PEGylation to the N-terminus of lysozyme in a site-specifi c manner was achieved using PEG-propionaldehyde derivatives with a molecular weight of Mw 20,000. A semi-preparative RP-HPLC method for the purifi cation of PEGylated lysozyme was developed using...

Graphite oxide (GO) has received extensive interest as a precursor for the bulk production of graphene-based materials. Here, the highly energetic nature of GO, noted from the self-propagating thermal deoxygenating reaction observed in solid state, is explored. Although the resulting graphene product is quite stable against combustion even in a natural gas fl ame, its thermal stability is signi...

Karlsson, K., 2004, Substance P Endopeptidase; Purifi cation and Characterization of Enzyme Activity and Evaluation of its Function during Stressful Condition, Acta Universitatis Upsaliensis, Comprehensive Summaries of Uppsala Dissertations from the Faculty of Medicine 1331, 56 pp. Uppsala. ISBN 91-554-5908-0. The purifi cation and biochemical characterization of the substance P (SP) hydrolyzin...

s of Papers, 232nd ACS Meeting, San Francisco, CA, 2006. Yigzaw, Y. , Piper, R. , Tran , M. , Shukla , A.A. ( 2006 ). Exploitation of the adsorptive properties of depth filters for host cell protein removal during monoclonal antibody purifi cation. Biotechnology Progress 22, 288–296. Roque, A.C.A. , Silva, C.S.O. , Taipa, M.A. (2007). Affi nit...